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Our lab aims to understand the molecular mechanisms of cell-cell signaling in development and diseases. Under supervision, the individual hired will primarily be involved in genotyping of mice necessary for management of the mouse colony. This work requires molecular biology techniques, especially PCR, gel electrophoresis of nucleic acid, and preparation and manipulation of DNA.Â
Responsibilities
DUTIES & ESSENTIAL JOB FUNCTIONS
Identify the functions or tasks that employees in the job perform. The essential functions should state the purpose of the work and the results to be accomplished, rather than how the function is performed. Of the tasks listed, what percentage of time is devoted to each? The more time employees spend on a function, the more likely it is that the function is essential. Generally, include those functions that account for 10% or more of the work, i.e., key items that contribute significantly to the achievement of the job. The functions should add up to 100%.
% Â of time | Essential Function (Yes/No) | Â Â Key Responsibilities (To be completed by Supervisor) |
80 | Yes | Under supervision, perform molecular biology/biochemistry • genomic DNA isolation • agarose gel electrophoresis • isolation of DNA from agarose gel • ligation of DNA • transformation of DNA • mini- and maxi-preparation of DNA • PCR |
20 | Yes | Under supervision, perform developmental biology • general mouse husbandry • mouse embryo dissection (including dissection of internal organs) • fixation, embedding, sectioning of mouse embryos • antibody staining of mouse embryos and adult tissues (whole mount and  section) • EdU and Tunnel staining of mouse embryos and adult tissues • staining skeleton for bone and cartilage of mouse embryos and adult mice • organ (embryonic mouse lung) culture |
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100% | Â | (To update total %, enter the amount of time in whole numbers (without the % symbol - e.g., 15, 20) then highlight the total sum (e.g., 1%) at the bottom of the column and press F9. The total sum should add up to 100%.) |
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Qualifications
Required Qualifications:
•    BA/BS in a related science and knowledge of, or experience with, the basic techniques or methods required by the position; and/or an equivalent combination of education and experience.
•    Attention to detail
•    Ability to follow instructions and protocols
•    Ability to keep track of animal numbers during PCR and gel electrophoresis
•    Time management skills to complete the assigned tasks within 16 hours
•    Excellent written and verbal communication skills
•    Excellent interpersonal skills
•    Excellent organizational skills to aid in the smooth operation of the laboratory by maintaining adequate supplies and equipment operation
•    Ability to handle unpleasant elements and wear protective clothing or equipment
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Preferred Qualifications:
•    Experience in immunostaining as acquired in school
•    Experience in tissue processing as acquired in school
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