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Scientist I (Gene Therapy/ Cell Cloning)

Dawar Consulting, Inc.
Posted 3 months ago, valid for 20 days
Location

San Diego, CA 92132, US

Salary

$46.43 per hour

Contract type

Contract

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Sonic Summary

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  • Our client, a leading company in life sciences and biotechnology, is seeking a Scientist I in San Diego, CA.
  • This long-term contract position offers a pay rate of $46.43 per hour on a W2 basis, along with benefits such as medical, dental, vision, paid sick leave, and 401K.
  • The Scientist I will focus on R&D for non-viral cell and gene therapy delivery, specifically evaluating novel mRNA and DNA designs using various molecular biology techniques.
  • Candidates must have a Bachelor's degree with 5+ years or a Master's degree with 2+ years of experience in molecular biology or a related field.
  • Required skills include advanced cloning techniques, functional assays, and cell culture experience, along with proficiency in software such as Snapgene and MS Office.

Our client, a world leader in life sciences and biotechnology, is looking for a “Scientist I” based out of San Diego, CA.

 

Job Duration: Long Term Contract (Possibility Of Extension)

Pay Rate : $46.43/hr on W2

 

Company Benefits: Medical, Dental, Vision, Paid Sick leave, 401K

 

The Scientist I will be a vital contributor to our non-viral cell and gene therapy delivery platform, primarily focused on R&D for innovative nucleic acid payload technologies. This role involves evaluating novel mRNA and DNA designs using a range of molecular biology techniques, including plasmid cloning, in vitro validation, and the production of in vitro-transcribed mRNA. To verify successful expression, the scientist will utilize assays such as Western blot, qRT-PCR, and luciferase reporter assays.

 

Job Responsibilities:

 

·       Design and clone expression vectors and IVT donor plasmids to support research initiatives.

·       Execute DNA and mRNA transfections in mouse and human cell lines.

·       Assess nucleic acid expression through molecular methods like qPCR, reporter assays and Western blotting.

·       Maintenance of detailed electronic laboratory notebooks (ELN) and preparation of summary slides

 

Required Qualifications:

  • Bachelor's degree with 5+ years or a Master's degree with 2+ years of experience in molecular biology, pharmaceutical sciences, or a related field.
  • Molecular Biology: Required proficiency in advanced cloning (Gibson assembly, Golden Gate, site-directed mutagenesis) and IVT.
  • Functional Assays: Hands-on expertise in measuring transgene delivery success via reporter assays and qRT-PCR.
  • Cell Culture: Preferred experience in genetic manipulation of cell lines and primary cells (e.g., T cells, monocytes, hepatocytes).
  • Desired proficiency in Snapgene, Prism, and MS Office.

 

 

 

If interested, please send us your updated resume at

hr@dawarconsulting.com/akansha@dawarconsulting.com

 






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